Grubska L. The structure-functional state of the mitochondrial inner membrane of the rats small intestine mucosa cells exposed to ionizing radiation with low intensity.

The dissertation is devoted to investigations of the structure-functional state of the mitochondrial inner membrane of the rats small intestine mucosa cells under the single exposed to ionizing radiation in the dose 0,1; 0,5 and 1 Gy with dose input 0,055 Gy/min were carried out. Animals were decapitated in 1, 12 and 24 hours after total irradiation. The discoupling of the oxidative phosphorylation, the disturbance functions of the electron-transport chain enzymes, the suppression of ATP-ase activity, the change of the contents of cytochroms groups: a, b and c and ubiquinone, the modification of the lipid and fatty acids composition of the mitochondrial inner membrane of small intestine mucosa cells were revealed. The main disturbance of the protein and the lipid components of the inner mitochondrial membrane under the conditions of the investigations were analyzed. The changes of the membrane-chain enzymes of the mitochondrial inner membrane and appropriate disturbance of their functions were established as the result of indirect effects by other components of membrane - lipids (the decrease of quantity of the phospholipids, the change of their composition what characterized by the predominance of unsaturated fatty acids, and the increase of cholesterol, at all), and by deficit of ATP and by the deenergization of the cells. The established structure-functional disturbances of the mitochondrial inner membrane of small intestine mucosa cells extend the existed presentation about the mechanism of the effects of ionizing radiation with low intensity action in the dose range 0,1-1,0 Gy. These investigations complement the methodological approaches on valuation of the radio-biological effects.