Didenko G. The designing of antitumor vaccines on the basis of protein-containing metabolites of B. subtilis В-7025 and their effects on the separate reactions of anti-tumor immunity (experimental study)

The dissertation is devoted to research of antitumor action of the new vaccines designed with use of products of a microbic origin. Object of research: Сytotoxic albuminous metabolits of Bacillus subtilis В-7025 and the cancer vaccines constructed with their use. The purpose: To develop the cancer vaccines prepared with use of exogenic cytotoxic metabolits of B.subtilis В-7025, to investigate their antineoplastic efficacy and influence on separate branches of immune system. Methods: use of model systems of tumor growth, cytologic, biochemical, immunologic, statistical. Methods: use of model systems of tumor growth, cytologic, biochemical, immunologic, statistical. It was obtained two exogen albuminous metabolits of B.subtilis В-7025 components (18,5 kDa and 70 kDa) and it was shown the cytotoxic action of these components on tumor cells іn vitro. The efficiency of the vaccines on various models of tumor growth (sarcoma 37, 3LL, L1210 and Ehrlich's cancer) in the experiments has been shown. The analysis of immune effects induced by vaccine therapy was suggested that realization of vaccines' antineoplastic action is carried out due to a high level of antibody-dependent reactions mediated by of macrophages and lymphocytes. The observed reactions were considered as a result of removal or blocking action of the serum at the terminal stages of experiment. Field of application is medicine.