Kosenko O. Gels systems based on acrylic monomers for stem cells immobilization

This dissertation is devoted to the colloidal-chemical investigations in stem cells bio-compliant acrylic hydrogels synthesis aspects regularitys and in subsequent development of bio-composites for burn wounds coverings. Acryl monomers radical copolymerization parameters for bio-compliant hydrogels synthesis were investigated, which leads to high water content, convenient strength-elasticity properties and nano-dimensional internal structure cells. It was established, that efficiency of immobilization and cultivation of stem cells depends on acrylic hydrogels composition and it can be improved by incorporation of high-dispersed silica in the internal structure. It was established, that acryl hydrogels adhesion of stem cells is defined by selective adsorption of aqueous-soluble exo-metabolites on hydrogels surface. Specified mechanism for hard-base heteroadagylation of stem cells was proposed. The correlation parameters were investigated and mechanism for hydrogels systems interactions with various nature solvents at different pH values of solvent and temperature was proposed. Investigations were also made for hydrogels sorption properties determining in relation to low-molecular and high-molecular compounds. The correlations between hydrogels composition and their mechanical and optical properties were established. The technological scheme creation of bio-composite wound coverings as artificial equivalent of skin with immobilized stem cells was proposed and clinical testing was provided