Kondratyuk N. Сomparison of Diagnostic Microbiological Methods Efficiency in Tuberculosis of Different Localization

Сomparison of Diagnostic Microbiological Methods Efficiency in Tuberculosis of Different Localization. The subject of research: disclosing, identification, and determination of resistance of the tuberculosis mycobacteria. The object of research: comparison of effectiveness using of the microbiological methods of diagnostics tuberculosis of different localization and determination of sensitiveness of detached mycobacteria tuberculosis to antimycobacterial preparation. Methods of research: microbiological, biochemical and statistical. It was ascertained , that at inoculation of pathological material with further cultivation in automated system Bactec MGIT-960, disclosing of the mycobacteria are on 10-15% more , than at the cultivation on hard nutrient surroundings of Levenstein-Jensen, and also in 1,5 times is a greater percentage the disclosing of mycobacteria at outlung forms, especially at lesion of nervous and osseous-joint system. Using the method of "enrichment", with previous treatment of pathological material by bicarbonate natrium solution, increases the percentage of disclosing tuberculosis mycobacteria in tubercular patient of urogenital localization. It is recommended to apply the modified test on nitrates reduction accordingly Barauskene for confirmation of the appurtenance excreted culture to mycobacteria of tuberculosis. It was proved the coincidence of results on tests of sensitivity MBT to ABS in Bactec MGT -960 system and by the method of absolute concentrations on the hard nutrient surroundings of Levenstein Jensen is high and on average forms 92,4 %. It was studied the resistance of tuberculosis exciter in case of outlung localization of the process. Area of use-microbiologic, phthisiology.