Kot Y. The Structural-metabolic response of the connective tissue to the action of an mechanical stretching

Mechanical pressure (0 - 0,5) N/m2) influence on synthesis, processing and concentration of collagen, elastin and glycosaminoglycans in the skin and aorta of rats, on visco-elastic properties of these organs, some intra- and internal sites of mechanical pressure signal transmission to the synthetic device of cells were researched. The methods of research. Radioisotopes analysis, analytic determination of collagens type 1 gexoses, aldehid- and ?-amino groups; ion-exchange chromatography, measurement of mechanical properties of skin and aorta, its elastic properties, differential scanning calorimetry, statistic methods. The results of study, scientific novelty. The synthetic intensity of investigated biopolymers was increased with the pressure growth, and after reaching the maximum, starts to decrease. The increase of the studied biopolymers synthesis intensity and concentration at physiological values of pressure; are adaptive cellular response to an external pressure. their decrease occurs atpressure that begin destroy the tissue structure, when synthetic possibilities of cells can be axhausted. The decrease in activity of oxidative deamination of lysine and oxylysine, of proline hydroxylase activity at mechanical pressure growth, took place . The degree of glycosylation of collagen at first increases, and having reached the maximum, decreases to the basal level. It is revealed, that protein-polysaccharide matrix formations represent an intercellular site of mechanical pressure signal transduction, and actin complex of the cytoskeleton and that of Са2+/calmodulin are its separate intracellular sites. The sphere of application: gerontology, sports medicine, orthopaedic medicine and surgery.