Trufanov O. The HT-2 toxin: microbiological method of detection, occurrence, toxicity and application of Bacillus subtilis preparations at HT-2 toxicosis of chickens

The object: toxicity of the HT-2 toxin; sensitiveness of C. pseudotropicalis, L. reticulatus and rabbit to the HT-2 toxin; contamination of forages by the HT-2 toxin; HT-2 toxicosis of chickens; the antitoxic action of B. subtilis. The aim: to develop the microbiological method of detection of the HT-2 toxin, to investigate its distribution in the Ukraine, to explore action of the HT-2 toxin on C. pseudotropicalis, L. reticulatus, rabbits and chickens and to define efficiency of application of the B. subtilis preparations at HT-2 toxicosis of chickens. Methods of studies: microbiological, biochemical, chromatographic, morphological, statistical, histological, photometric, disk-diffusion method, thin-layer and column chromatography, bioautography, spectroscopy of nuclear magnetic resonance. The toxicity of the HT-2 toxin for C. pseudotropicalis, L. reticulatus and rabbits was defined. The synergism of action of naphthalene derivatives and trichothecenes on C. pseudotropicalis was discovered. The microbiological method for HT-2 toxin detection was developed. The HT-2 toxin was detected in 6,8% from 864 explored samples. Probiotic preparations of B. subtilis had prophylactic effect on chickens during chronic intoxication by the HT-2 toxin, which declare itself in oral lesions, bourse dystrophy, delay of growth, decreasing of laying ability and imbalance in biochemical composition of eggs, hypertrophy of internal organs, decrease of concentration of total protein and increase of uric acid and creatinine concentration in blood plasma.Field of application: microbiology, ecology, poultry, veterinary medicine.