Kit L. Purple sulfur bacteria from Yavoriv water reservoirs of sulfuric deposits: morfophysiological characteristic and their role in hydrogen sulfide detoxication

Object of research: process of hydrogen sulphide oxidation by anoxygenic photosynthetic sulfur bacteria. The aim of work: realization of ecological-microbiological monitoring of water reservoirs of Yavoriv sulfur deposit, isolation and identification of pure cultures of photosynthetic sulfur bacteria, studying of their role in processes of water purification from hydrogen sulphide. Methods of researches: microbiological, cytologic, biochemical, physical and chemical, chemical, statistical. It has been shown that saprophytic and oligonitrophylic microorganisms dominate in microflora of water reservoirs of Yavoriv sulfur deposit. As well there has been revealed gradual increase of amount of photosynthetic sulfur bacteria, which have a typical seasonal change. Methods of isolation and cultivation of these bacteria have been improved. The pure cultures of purple sulfur bacteria have been isolated from water reservoirs of Yavoriv sulfur deposit. These bacteria have been identified as Thiocapsa sp., Lamprocystis sp. and Chromatium sp. Qualitative and quantitative composition of the main photosynthetic pigments of purple sulfur bacteria has been carried out. The physiological especially of these bacteria have been investigated. As well it was denoted that purple sulfur bacteria are capable of active utilization of hydrogen sulphide, and can play an important role in detoxication of the mentioned polluter in water reservoirs of Yavoriv sulfur deposit. Sphere of use: applied microbiology, ecology, nature conservation.