Zubov D. In vitro osteogenic induction and immune properties of mesenchymal stem cells

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0409U002247

Thesis Registration Form

0409U002247.pdf

Applicant for

Specialization

  • 03.00.09 - Імунологія

28-04-2009

Specialized Academic Board

Д 26.001.14

Taras Shevchenko National University of Kyiv

Essay

The investigations on osteoimmune interactions result that experimental mesenchymal stem cell (MSC) groups (uncommitted and committed osteogenic) express СD44, СD166, СD58, CD62L, CD29, CD49b, CD49c and CD54 molecules. There is no expression of СD49a and HLA A, B, C molecules. Committed group of MSC begins to express HLA-DR molecules on a single cell level (p<0,05) and stops to express CD56 (p<0,05). Cultured committed osteogenic MSC begin to product the alkaline phosphatase (AP) from the 10-th day of osteogenic induction. Maximal number of AP-positive МSC cultures was observed on days of 13-19 of osteoinduction and consists of 90%. The cytokine profile consisting of IL-1 beta, IL-2, IL-4, IL-6, IL-8 and TNF-alpha was secreted by both experimental MSC groups. The secretion of IL-1 beta and IL-2 by cultured MSC together with over-production of IL-6 (up to 276,5 pg/ml, р<0,05) and IL-8 (up to 106,6 ng/ml, р<0,05) by osteogenic inducted MSC are firstly shown.

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