Iakovenko O. Role of phospholipase C in abscisic acid action mechanism

Phosphoinositide-specific phospholipase C of plants cells as a component of an alarm way abscisic acids in cells of plants. Research of participation phosphoinositide-specific phospholipase C in signal transduction abscisic acids in cells of plants in vivo. Tin liquid chromatography, radioisotope methods, autoradiography, light microscopy. The activation of phospholipase C during early stage of ABA action was elucidating.The contribution of PI-PLC to ABA signaling in guard cell was investigated by PI-PLC inhibitor neomycin treatment. ABA promotes an open stomata closing and inhibits the light-induced opening of closed stomata. Aperture size of stomata epidermal peels treated with ABA combined with neomycin was increased compared to control level. Exogenously added neomycin induced stomata opening, reduced stomata closing by ABA and inhibited ABA ability to suppress stomata opening. The pretreatment with neomycin impair stomata sensitiveness to ABA signal. Furthermore guard cells of epidermis treated by ABA were not sensitive to neomycin. The above-mentioned results indicate that PI-PLC plays a role in the signaling events associated with the inhibition of stomata opening by ABA and cellular reactions that are responsible for ABA-induced stomata closure. Sphere of use: bioorganic chemistry, physiology of plants.