Kovalevska L. Expression of protein kinase D2 in normal and malignant human B lymphocytes.

The work was aimed on the study of expression? autophosphorylation and intracellular localization of protein kinase D2 (PKD2) in norma and malignant human B lymphocytes. We found that the PKD2? but not PKD1? is expressed on mRNA and protein level in normal and malignant B cells. The level of PKD2 expression and autophosphorylation was studied in different subpopulations of normal mature B cells. Our studies indicate that PKD2 is a downstream target of BCR signals in normal И lymphocytes, as well as in malignant И cells. BCR activation initiates PKD2 autophosphorylation and progressive sustained nuclear relocalization in primary normal B lymphocytes. Using biopsies of primary tumor we showed that tumor cells in small lymphocytic lymphoma, mantle cell lymphoma and sporadic Burkitt's lymphoma had the moderate level of PKD2 expression and low level PKD2 uatophosphorylation. The high level of PKD2 expression and autophosphorylationwas found in Reed-Sternberg cells in classical Hodgkin's lymphoma.