Kolodiy S. Microbiological grounding of nutritious environment development and usage for tuberculosis mycobacteria cultivation

A research object is cultures of tests of Mycobacterium tuberculosis H37 RV, Mycobacterium tuberculosis (resistant to antiphthisic preparations), Mycobacterium tuberculosis (sensible to antiphthisic preparations), Mycobacterium bovis and them biological description. Research purpose: a microbiological ground of effective application of new methods of diagnostics and nourishing environments is for the selection of Mycobacterium tuberculosis from biological material. Research methods: microbiological, physical and chemical, mathematic and statistic. Apparatus: binocular microscope MIKMED-1, microscope MBR-1 with the video camera Quick Cam Home. It is well-proven that 0,1% solutions of antiseptics of decametoxinum, myramistinum does not repress growth and reproduction of mycobacterium and own high decontaminant activity in relation to a concomitant microflora. Grounded application of new effective nourishing environment for microbiological diagnostics of tuberculosis. The deep study of biological properties of exciter of tuberculosis is first conducted. It is discovered that in the process of vital functions of mycobacterium need enhanceable maintenance of linolic acid and iron. Grounded method of microbiological diagnostics of tuberculosis for introduction in the network of treatment and prevention antiphthisic establishments. Three patents are got on useful models on perfection of nourishing environment and method of selection of exciter to tuberculosis. Methodical recommendations of Ministry of Health of Ukraine are developed: The modern going to diagnostics of tuberculosis. - K, 2006. Application domain: medicine, veterinary science.