Kharchenko O. Biochemical mechanisms of chronic alcoholic intoxication development and under acetic zinc action

Ethanol metabolism systems functioning (alcohol dehydrogenase, aldehyde dehydrogenase activity, cytochrome Р450 content), peroxidation processes (content of TBC-products, diene conjugate, shif bases), antioxidative systems (SОD, catalase, glutathione-dependent enzymes activity), structural and functional condition of plasmatic membranes of rats liver and brain cells, blood serum and certain indicators of the immune status (2',5'-oligoadenylatesynthetase activity, interleukin 1 6 content) were investigated under the chronic alcoholic intoxication and also was established influence of acetic zinc on these parameters. It was shown, that under the chronic alcoholic intoxication there was dysfunction of the basic systems of ethanol metabolism through the decrease of system ADG/AlDG activity and activation of microsomal oxidations (increase of the cytochrome Р450 content). The gradual increase of general NO-synthase activity was established in rats liver and brain cells under chronic alcoholic intoxication. The disturbance of systems of the immune answer formation (decrease of splenocyte 2',5'-oligoadenylate-synthetase activity, increase interleukin 1 6 content in blood whey) was established under conditions of alcoholic intoxication development. The features of the phospholipids content reduction and the increase of basic membrane-bound enzymes activity (Na+,K+-АTPase, Ca2+,Mg2+-АTPase and 5'- nucleotidase) of plasmatic membranes of rats liver and brain cells was characterized under chronic alcoholic intoxication. The accumulation of toxic lipid peroxidation products (TBC-products, diene conjugates, sheef bases) with the simultaneous decrease of antioxidative enzymes activity (SОD, catalase, glutathione-dependent enzymes) in rats liver and brain cells and serum during the chronic alcoholic intoxication. The acetic zinc treatment resulted in the reduction of ethanol influence on investigated metabolism indicators (normalization of the membrane phospholipids, cytochrome Р450, lipid peroxidation products content; SOD, catalase, glutathione-dependent enzymes, NO-synthase activity; decrease of membrane-bound enzymes activities, increase of ADG and AlDG activities) in rats liver and brain cells and serum in comparison with animals with a chronic alcoholic intoxication.