Lyaskovsky T. Microflora of pregnant women and methods of it's correction at disbiosysis

The candidate degree thesis by specialty 03.00.07 - microbiology.- Іinstitute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv, 2009. Quantitative and genus stuff of vaginal microflora and intestine of pregnant women have been examined. The study of main taxonomic groups of microorganisms isolated shows the presence of lactic acid bacteria in vaginal in the amount of secretion 105-108 CFU/0,1ml and 107-108 CFU/1g in the intestine specimen. The E.coli presence was at concentration 102-103 і 106-107 accordingly. The yeast presence was at concentration 102-105 in vaginal secretion and 103-104 in the intestine specimen. Pathogenic microflora was absent. Lactic acid bacteria were represented by Lactobacillus acidophilus, L. plantarum, L. fermentum, L. vaginalis; Enterococcus faecium, E. faecalis, E. durans; Streptococcus thermophilus and Lactococcus lactis. Identification of probiotic strains of lactic acid bacteria based on phenotype characteristics (API-testing) and molecular biology techniques with the use of specific primers confirmed the genus of Streptococcus thermophilus, Enterococcus faecium, Lactobacillus plantarum. The antagonistic properties of probiotic cultures of lactic acid bacteria towards referent strains of pathogenic microorganisms have been shown. Antagonistic activity depends on the genus and strain of lactobacilli as well as conditions of cultivations. Adhesive activity of probiotic strains of lactic acid bacteria to vaginal epithelium have been also shown. For the first time, the change of antibiotics sensitivity of lactic acid bacteria on the model of lymphoblastoid cells have been indicated. It have been exhibited the ability of probiotic strains to induce alfa and gamma interferons, as well as to inhibit flu and genital herpes viruses activity. Probiotic substance have been proposed for the purpose of use as prophylactic and curing preparations for vaginal and intestinal disbacteriouses treatment.