Rotar D. Intestinal microflora and its translocation in internal organs of experimental animals with acute pancreatitis

Object of research: anaerobic and aerobic microorganisms, which are presented in intestine of experimental animals during acute pancreatitis. Purpose - to establish changes of species composition and population level of luminal and mucosal microflora of large and distal part of small bowel and their influence on frequency and level of bacterial translocation into pancreas and others biotopes during acute pancreatitis. Methods: microbiological, morphological, histological, statistical. Equipment: nutrient mediums, thermostat, anaerostat, microscope. Theoretical and practical results: It was difined that the sequence and degree of bacterial contamination of pancreas and other internal organs are depended from the severity of acute pancreatitis and changes of intestinal microflora. Changes of species composition and population level of luminal microflora of large intestine during acute destructive pancreatitis was proposed to use as criterion of prediction of pancreatic infection. Novelty: it was established, that the acute pancreatitis is accompanied by changes of luminal and mucosal microbiota of large and distal part of small bowel which degree are depended from severity of pancreatitis. It is proved, that excessive mucosal bacterial colonisation of intestines increases permeability of intestinal wall and enhances bacterial translocation from gut into portal blood, peritoneal cavity and internal organs. Introduction degree: in educational process of profile chairs MH of Ukraine; 1 innovation №391/31. Area of use: medicine (surgery).