Isaenko O. Biological characteristic of antigenes of B.pertussis obtained with the help of frequency ultrasound

Object of the investigation: industrial cultures Bordetella pertussis № 267 and № 475, fractions of disintegrates after the action of a low-frequency (60 кHz), a mid-frequency (130 кHz) and a high-frequency (1,6 MHz) ultrasound, redblood cell , laboratory animals (mice, porpoises, rabbits). The aim: to prove a technique of the antigens B.pertussis reception experimentally by means of physical methods and to define perspective fractions for acellular antipertussis vaccines improvement. Methods of the investigation: microbiological, biochemical, immunological, statistic. Equipment: ultrasonic generators (Г3-109, TУ 3468-001-42369179-03, УД-1), gel-chromatograph, biochemical analyzer, pH-meter, analytical scales, laboratory centrifuge, ultracentrifuge, microscope "Biolam lоmо". The receiving native low-molecular antigens technique is proved at preservation of bacteria B.pertussis viability, which can be applied for reusable disintegration of superficial fractions at continuous cultivation of microbe weight in productive processes. For the first time by means of ultrasonic decomposition (60 кHz, 5 Vt, 7hours) and gel-chromatograph fractionating they allocated the antigen with molecular weight ~ 8,1 кDа, which possesses high protective activity and which is free of toxic, dermonecrotic, histamine sensitization properties and high-molecular fraction ? 1000 кDа which is free of toxic, histamine sensitization, dermonecrotic properties, immunization properties oremarked bodly, but it reduces adhesion properties B.pertussis. This can have a great value for prevention of development of a primary stage pathological process of a whooping cough. The level of the introduction: application of the patent, the methodicals recommendations, two innovations, new technology. The field of usage: medicine (the enterprises on release immunobiological preparations).