Oberemok V. DNA markers in the іnvestigation of the interaction between Lymantria dispar multiple nucleopolyhedrosis virus and its host gypsy moth

Thе thesis is dedicated to the application of the DNA markers in the study of the interaction between Lymantria dispar multiple nucleocapsid nuclear polyhedrosis virus and its host gypsy moth. Transovarial transmission of virus is revealed using RAPD-PCR and primer OPA-08 and PCR with specific primers. It is discovered that the single chain fragments of virus DNA (gene inhibitor of host cell apoptosis, iap-3 gene) increase the death rate of gypsy moth caterpillars when wafted outwardly. With the help of RAPD-PCR and primer OPA-14 genetic difference between gypsy moth individuals correlating with the development of virus reproduction is found. The low intensity of accumulation of virus polyhedra is marked by the fragments of virus DNA with the length of approximately 150, 430, 850 and 1000 b.p. and high intensity of virus accumulation is marked by the DNA fragments with the length of approximately 330 and 560 b.p.