Kostytska O. Foundation of facility of using of cultured intervertebral disc cells for treatment of patients with spine degenerative diseases.

Object: intervertebral disc regeneration using cultured cells. Aim: ground-based experimental research of the possibility of using cultured cells of the intervertebral disc in patients with degenerative spine disease. Methods: clinical, radiological, experimental, statistical. It is experimentally proved the possibility of using cultured autologous and allogeneic cells of the intervertebral disc to optimize reparation. It is shown that transplantation of autologous cells derived from the intervertebral disc of young rats in areas of his injury is accompanied by activation of reparative processes and formation hondroyid that contacts with fibrous annulus without clear boundaries. Ultrastructure of cells regenerate shows their cartilage phenotype and high biosynthetic activity. Reparative processes in the intervertebral discs of old rats with transplantive of autologic cells, do not ensure full recovery of the damaged intervertebral disc structure, which is due to the influence of age changes in it, and low proliferative and biosynthetic capacity of cells. For the first time shown, that transplantation of cultured allogeneic cells of the intervertebral disc of young animals in the II damaged disc area of old animals stops development of destructive changes and optimizes its repair. Found, that for transplantation of cultured cells in the damaged disk area ist important the current number of cells and the dynamic neutralization of the vertebral segment. There are new knowledge about the features of differentiation, cell proliferation and biosynthesis of fibrous annulus and nucleus pulposus of intervertebral disc in the joint cultivation in different types of the cultivated cells of young and old rats. Found, that high proliferative and biosynthetic activity of cells in culture, preserve their phenotype provides a significant percentage of the density of cells and the microenvironment in the form of matrix macromolecules formed during cultivation. First proved, that the cells of intervertebral discs in old rats are characterized by low proliferation and low biosynthetic activity. The formation of intercellular matrix components in the same conditions of cultivation seen only in cell cultures of young animals. Found, that collagenic microenvironment of intervertebral disc cells cultivated on matrices, creating conditions similar to the existence of cells in vivo so as to preserve the viability of cells, their phenotype and active proliferation. Ultrastructural organization of cells indicates their essential metabolic potential: in the intercellular space granules accumulate proteoglycans and collagen type II fibrils in joint cultivation of cells and fibrous annulus of nucleus pulposus, and cultivated cells fibrous annulus of intervertebral disc - collagen type I and II. The application of autologous and alohenyh cultured cells of the intervertebral disc leads to improvement the results of treatment by optimizing the reparative processes. The proposed method of cultivation of cells (patent of Ukraine № 24 201) able to get enough them for implantation on collagen matrix. Compliance with requirements of this method in clinical practice will ensure the maximal effect of transplantation of cells in the disc in patients with degenerative spine. The study is introduced in the educational process and research work at the Department of Human Anatomy of Lugansk State Medical University, Department of General Surgery with courses surgery, operative surgery and forensic medicine "Uzhgorod National University", Department of Orthopedics and Traumatology HMAPS, neuropathology and neurosurgery department of the Lviv National Medical University, Department of Orthopedics and Traumatology, Ivano-Frankivsk National Medical University. Traumatology and orthopaedy.