Taran O. Regenerating ability of potato plants under the influence of abiotic factors in vitro and ex vitro culture

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0411U003652

Applicant for

Specialization

  • 03.00.12 - Фізіологія рослин

30-05-2011

Specialized Academic Board

Д 26.001.24

Taras Shevchenko National University of Kyiv

Essay

Influence of light of different spectral structure on different modeling objects in culture of a potato in vitro for the first time is investigated. Increase of reclaiming ability of explants and regenerants in culture in vitro under the influence of this factor is revealed. New data concerning influence of light of red area of a spectrum on contents of phytogormons in potato regenerants are obtained. Increase of reclaiming ability of cuttings of a potato in conditions ех vitro at use new is proved a substratum for cultivation and application of brassinosteroid. Simulated microgravitation on a reproduction of viruses of different taxonomic groups influence research is carried out potato plants for the first time. Decrease in the maintenance of antigens of viruses in fabrics of plants of a potato under the influence of a long clinostating is revealed. Existence of systems "phytovirus - host plant" with various gravisensitivity is established. At application of a combination of methods simulated microgravitation and culture apical meristems it is received regenerants in vitro, free from a virus infection. As a result of researches parameters of optimisation of technological process of cultivation of a potato in conditions in vitro are found out. The way of adaptation of microtubers of a potato and a way of adaptation of regenerants of culture in vitro to conditions ех vitrо is offered. Parameters of cultivation of plants in culture ех vitro are developed, the new substratum for cultivation of nodal cuttings from regenerants in vitro in culture ех vitro is offered. It is proved approaches for working out of methods of improvement of plants with application of ecologically safe receptions, in particular, with microgravitation use.

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