Medynska K. Action of ultrasound of different intensities on structural and functional state of skeletal muscles actomyosin in vitro

Object: structural and functional characteristics of rabbit skeletal muscles actomyosin. Aim: to define influence of ultrasound of different modes and intensities on structural and functional characteristics of intact and oxidized rabbit skeletal muscles actomyosin. Methods: preparative protein chemistry, electrophoresis, spectrophotometry (determination of ATP activity, registration of superprecipitation reaction of actomyosin), fluorescent spectroscopy, oxidized modification of proteins, kinetic analysis (calculation of enzymatic reaction characteristic constants and parameters of superprecipitation). Results: ultrasound of therapeutic range influences on ATP activity, parameters of superprecipitation reaction and position of fluorescence spectrum of native and oxidized actomyosin complex and some its components. Novelty: influence of ultrasound of different modes and intensities on the structural and functional properties of native rabbit skeletal actomyosin has been studied at the first time; the results of reconstructed actomiosin research suggest that troponin complex is the most ultrosound sensitive constituent of the actomyosin; it has been shown that action of therapeutic range ultrasound does not result in normalization of spectral characteristics and functional activity of actomyosin damaged by free radicals. Area of application: biophysics, biochemistry.