Fomenko O. The content, tissue distribution and biochemical properties of neuron- and astrocyte-specific proteins in experimental encephalopathy

The changes of neuron and astrocyte proteins (Ca-binding protein S100b, glial fibrillar acid protein, neuronal cell adhesion molecule) level and total GAG-binding extracellular matrix protein ability in the rat brain under hepatic encephalopathy condition and their correction were studied. Methionine-choline deficient non-alcoholic steatohepatitis is accompanied by diminution of neurospesific proteins, degradation of glial cells and decreasing of the amount of adhesive molecules; the cerebellum is the most staggered brain part. Experimental chronic hepatitis is accompanied by the astrocytes edema and increasing of the amount of adhesive molecules as well as neurospesific proteins, the cerebellum and hippocampus have more strong changes. At the development of experimental chronic hepatitis there was direct cross-correlation dependence between the locomotor's animal activity and the filament GFAP level (r = 0,86) and the mean reverse cross-correlation dependence between the cytosolic GFAP level and the locomotor's animal activity (r = -0,43). The reverse cross-correlation dependence between rat's cognitive activity and S100b level (r = -0,72), cytosolic GFAP level (r = -0,64) and direct cross-correlation dependence between the membrane NCAM level and level of cognitive activity (r = 0,74) was also set. There is direct cross-correlation dependence (r = 0,5-0,86) between the blood serum S100b level and S100b level in a cerebellum at the hepatic encephalopathy. Administration of Glutarginum, Cytoflavin and intermediate metabolite alpha-ketoglutarate prevented the development of neurospecific proteins changes in the rat's brain. Alpha-ketoglutarate and Cytoflavin were more effective. Key words: S100b, GFAP, NCAM, GAG-binding proteins, NASH, chronic hepatitis, hepatic encephalopathy.