Moravska O. Lipids and antioxidative status of geese tissues and embryos and their correction by vitamins A, D3 і Е.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0411U006618

Applicant for

Specialization

  • 03.00.04 - Біохімія

20-09-2011

Specialized Academic Board

Д 35.368.01

Institute of Animal Biology of NAAS

Essay

Object - an exchange lipids and per oxidations processes in a yolk of eggs and fabrics of embryos and a calcium -phosphoric exchange in fabrics of embryos and geese nestlings under influence of separate and complex action of vitamins A, D3 and E. Purpose - to find out influence of additional introduction in a diet of geese during the reproductive period of vitamins A, D3 and Е on lipids structure and antioxidative status, and also on processes of a calcium -phosphoric exchange in fabrics of embryos and geese nestlings. Methods: biochemical (spectrophotometers, colorimeters, chromatography), zootechnical, statistical. Novelty: for the first time is established an optimum level of vitamin Е in a diet of breeding geese during the reproductive period and his influence on a metabolism lipids and processes peroxidations lipids, and also increase vitamins A and E in fabrics of embryos. For the first time separate and complex introduction of vitamins A, D3 and Е in a diet of geese during the reproductive period adjusting action is established on increase of vitamin A, redistribution of separate classes lipids both their fat - acid structure and a level of products the peroxidations lipids in a yolk of eggs, a residual yolk and fabrics of embryos, and also on the contents of calcium, phosphorus and activity alkaline fosfataze in blood of embryos and geese nestlings. Results: it is determined that the growth of vitamin E dozes in a diet of geese during the reproductive period regulates the increase of vitamin A, E and leads to decrease in general lipids saturated and monononsaturated fat acids with growth of the content of polynonsaturated fat acids and also the decrease of per oxidations lipids in an egg yolk, fabrics and residual yolk of embryos. On the basis of the specified experiment it has been investigated an optimum doze of vitamin Е in a diet of geese during the reproductive period – is 35 MU of the vitamin E per kilo of mixed fodder. It has been proved that the compatible adding of vitamins in the proportion А – 10000 МU, D3 – 3000 МU and Е – 35 МU to mixed fodder of geese during the reproductive period reveals the most obvious effect in stabilization of a product level of peroxidations lipids and change of their fatty-acids structure, with growth of the content of polynonsaturated fat acids in the egg yolk of geese, fabrics and residual yolk of embryos. Also, the regulation of calcium-phosphoric exchange with the growth of the calcium and phosphoric amount and the reduction of alkaline phosfatase in the blood of embryos and geese is observed. Sphere of use: biochemistry, science about birds.

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