Dukh О. Antioxidant state and lipid composition of hens' and embryos' tissue under the nutritional correction by vitamin A and carotenoids

The object is the activity of ferments of the system of antioxidant defense and lipid composition of Shaver-579 hens' and embryos' tissue under the nutritional correction by vitamin A and carotenoids. The aim is to find out the influence of vitamin A and carotenoids on the contents of lipids and fatty acids, the amount of LPO products and the activity of the ferments of antioxidant system in hens' and embryos' tissue. Methods: biochemical (spectrophotometers, colorimeters, chromatography), zootechnical, statistical. Novelty: for the first time the influence of different levels of vitamin A and carotenoids in hens' combined diet in the reproductive period on the contents of lipids and antioxidant state of hens' and embryos' tissue has been researched. It has been established that increasing of vitamin A amount in hens' combined diet in the reproductive period from 10 000 to 40 000 ІО and of carotenoids from 8 to 32 mg per kilogram of combined diet per day increases the amount of phospholipids and total lipids in hens' and embryos' blood and liver, increases the amount of nonsaturated and decreases the amount of saturated fatty acids in total lipids amount, decreases the amount of LPO products in hens' and embryos' blood and liver, increases the activity of SOD and Catalase and the level of Ceruloplasmin. For the first time the optimal amount of vitamin A and carotenoids in hens' diet in reproductive period has been pointed out, and their influence on the accumulation of vitamin A and tocopherol in egg-yolks, hens' tissue and embryos, and also on hatchability, has been shown. The sphere of use: biochemistry, poultry breeding.