Artamonova A. Evaluation of the effectiveness of antitumor polysaccharide fractions from the fruit body of the fungus Lentinus edodes and glycopeptide vaccine in various experimental tumor models

Polysaccharide fraction from the fruit body of the fungus Lentinus edodes (PSF L.edodes) has been isolated and characterized. Antitumor and antimetastatic effect of PSFLE and glycopeptide cancer vaccine (GCV) has been analyzed in different experimental models of tumor growth: Ehrlich carcinoma, sarcoma 37, lymphoid leukemia L1210, Lewis lung carcinoma, melanoma B16. It has been revealed that one of the mechanisms of action of GCV and PSFLE is realized indirectly through the immune system. PSFLE and GCV increased cytolytic activity of lymphocytes, metabolic activity of peritoneal macrophages (MP) in the NBT-test, phagocytic activity of MP, cytolytic activity of blood serum in the presence of complement, the level of circulating immune complexes in blood serum of intact animals and animals with subcutaneously transplanted sarcoma 37. It has been shown that the combined use of the GCV and PSFLE resulted in the decrease of metastasis rate and primary tumor growth, and the increase of life-span of animals with transplanted tumors: sarcoma 37, Lewis lung carcinoma, and melanoma B16. In addition, such treatment resulted in significant elevation of the cytolytic activity of lymphocytes, the level of immune complexes in blood serum, metabolic activity of peritoneal MP in NBT-test, phagocytic activity of MP, the complement dependent cytolytic activity of blood serum in intact animals and animals with subcutaneously transplanted sarcoma 37.