Frych N. Antimicrobial and antimutagenic activity of Ericaceae plants extracts

Object of research: collection and clinical strains of gram-positive, gram-negative bacteria and fungi of the genus Candida, isolated from patients with purulent-septic and urological infections; strains of Salmonella typhimurium TA98 and TA100 (Ames test) and plant test (Allium cepa L.). Aim: Based on the microbiological and cytogenetic studies to evaluate the possibility of Ericaceae plants as a raw material for new effective antimicrobial agents and prevention of adverse genetic effects of environmental pollution. Methods: microbiological, genetic, cytological, mathematical. Theoretical and practical results: theoretical generalization and new address important scientific task - to overcome antibiotic resistance. The etiological structure of pathogens of purulent infections, wound infections and urinary infections of Ivano-Frankivsk hospitals and Ivano-Frankivsk region is dominated by gold and coagulasonegative staphylococci was established. The efficiency of using of Allium cepa-test and Ames test to assess the ability of plant extracts to restore chromosome stability, damaged by salts of heavy metals was shown. The experimental results indicate about the lack of genotoxic properties in plants water and ethanol extracts and justify the possibility of their use as antimutagenic funds. The scientific novelty: antimicrobial activity of plant extracts was set. Comparative analysis showed that among the Ericaceae plants the highest antibacterial activity is characterized for bearberry. 90 % aqueous ethanol extract of bearberry leaves have the ability to neutralize the usual classical mechanism of staphylococci meticylinresistancy and restore their sensitivity to protected penicillins and cephalosporins. For the first time in bacterial rapid tests the mutagenic and gene-protecting properties of water-ethanol extracts of Ericaceae plants were studied. Was shown that water-ethanol extracts of these plants, including bearberry don't have mutagenic activity and show a stimulating effect on growth and cell division of Allium cepa apical meristem. Scope: this study should widely publicize in the seminars and conferences, to include in the curriculum of ecologists, microbiologists, physicians and pharmacists. They should be implemented in the clinical work of medical institutions.