Artiushenko T. Participation of ascorbic acid and its metabolism enzymes in pea and maize physiological adaptation to the joint action of nickel and cadmium compounds.

functioning of ascorbate-depended antioxidant system at plants at cadmium and nickel compounds joint action and growth regulators influence. It is shown that cadmium and nickel ions absorption by roots has two-phase character. The most intensive metals adsorption takes a place at first 7 hours of a stress influence, and then - significantly goes down. The nickel and cadmium translocation to the pea leaves is noted at first hour of a stress action, while at the maize - after seventh. It is set that the cadmium and nickel compounds joint action induces oxidative stress which shows up in the increase of maintenance as dien, triene conjugates and TBA-active products compounds. The quantity of the last can be using as the stress influence markers. Participating of ascorbic acid in stabilizing of plants pro-/antioxidative balance at the actions of cadmium and nickel compounds shows up in the increase of its maintenance in leaves and roots for the first 7 hours, and after 12 hours - decline as a result of the intensive utilizing for the free-radical lipids peroxidation processes braking, on what indicates growth of dehydroascorbate and 2.3-dicetogulonic acids level. The features of ascorbate peroxidase and dehydroascorbate reductase functioning are first set at the joint action of nickel and cadmium ions, which are conditioned the accumulation of the last and species-specificity maintenance of ascorbate forms. Better adaptation ability of maize, as compared to a pea, will be realized due to more effective ascorbic acid oxidation/reduction enzymes functioning. It is first shown that previous treatment by the growth regulator Zeastimulin reduces the cadmium and nickel ions translocation to the maize leaves, while at the pea Agrostimulin use did not affect rates of its accumulation in leaves. Application of growth regulators resulted in the decline of lipids peroxidation products amount during intensification of ascorbate-depended antioxidant system functioning (an increase of ascorbic acid content and ascorbate peroxidase activity in leaves).