Ilyina Y. Efficacy of limbal autologous cells in treatment of recurrent herpetic keratitis, complicated by limbal insufficiency

Object: recurrent herpetic keratitis, complicated by limbal insufficiency. The purpose: the thesis is devoted to the treatment of recurrent herpetic keratitis, сomplicated by limbal insufficiency by transplantation of autologous cells with limbokeratofilling and conjunctival immobilization. Methods: confocal microscopy, biomicroscopy with a fluorescein stainning, algesimetry of the cornea, visometry, morphology of corneas of experimental animals; slit lamp, algesimeter, confocal tomograph HRT. Supplemented data that limbal insufficiency is associated with changes in neurotrophic stroma in case of recurrent herpetic keratitis, as proliferative activity of limbus epitheliocytes is the same at herpetic neurotrophic keratopathy, stromal megaherpetic keratitis and in healthy eyes. The method of autologous cells transplantation was worked out (limbokeratofilling), when limbal subepithelial flap was formed in injured eye, with following implantation of limbal biopsy material from the healthy eye. The method of limbal autologous cells trans-plantation was worked out (conjunctival immobilization). The proposed methods allow to improve the patient's treatment efficacy with recurrent herpetic keratitis due to complete regeneration of the damaged eye epithelium, limbal insufficiency compensation for up to 2 years and reduced the frequency of neurotrophic keratopathy and stromal herpetic keratitis. Results of work are introduced in Kharkov city hospital № 14, Donetsk regional clinical trauma hospital,the Kharkov and Lugansk regional clinical hospitals. The sphere of use - medicine, ophthalmology.