Zaychenko Y. Immune-microelementary associations and implications of dermatopathy in children residing in different areas of Lviv Region.

The dissertation work is based on analysis of the survey results of 170 children, aged 7 to 11 years (boys - 45.3%, girls - 54.7%), who resided in different areas of Lviv Region.Based on the mathematical model we established a direct relationship between accumulation of trace elements (lead, nickel) in hair of children and rising concentrations of these trace elements in soil and water. The prevalence of IgE-dependent dermatitis in children who lived in APA was 34% higher when compared with children who lived in ACA which associated with absolute and relative eosinophilia and a high content of allergen-induced trace elements like manganese, chromium and lead. In all children, regardless of residence area, we found likely (P <0.05) acceleration of erythrocyte sedimentation rate, likely (P <0.001) decrease in erythrocyte count and hemoglobin level. In addition, in children who lived in APA we observed a likely decrease of the relative number of segmented neutrophils, relative and absolute number of monocytes against the likely-increased of absolute and relative number of lymphocytes and eosinophils. In children residing in APA we observed likely increases of lymphocyte-granulocyte intoxication index and the index ratio of lymphocytes to monocytes, regardless of clinically-manifested dermatopathy. Based on mathematical model we determined the correlation relationship between higher concentration of lead in hair of children and risk of anemia, absolute lymphocytosis, and thus increased probability of immunopathology formation. In children from APA with the dystrophic changes syndrome, especially with dermatosis, we established the likely increase of the absolute number of T-lymphocytes and their subpopulations (CD4+, CD8+) and B lymphocytes compared to children who lived in ACA and the control group (P <0.001). In children who lived in APA we have seen a likely increase of IL-2 and IL 5, whereby the concentration of IL-5 was 1.8 times higher in children with dermatoses in comparison to children with verified dystrophic change syndrome. Multiple correlation regression model revealed that in hair of children living in APA the accumulation of xenobiotics of lead and manganese has increased the number of CD4+ lymphocytes (P <0.05), while the accumulation of xenobiotics of lead and nickel increased the number of CD3+ lymphocytes (P <0.05). Thus in children with clinical manifestations of the dystrophic changes syndrome, compare to those with dermatoses, we have seen less pronounced laboratorical and immunological changes that correlated with lesser accumulation of trace elements in the body.