Talokha N. Metabolic activity of microorganisms in the rumen of cattle at different concentrations of Pb, cadmium and chromium (VI) and ways of its correction.

Object - enzymatic processes in the rumen, the intensity of metabolism of carbohydrates and proteins for the actions of Pb, cadmium and chromium (VI). Purpose - To find out the toxic effects of Pb, cadmium and chromium (VI) on the physiological state of the organism bulls, metabolic processes in the rumen and find ways of its correction. Methods and apparatus - biochemical, clinical, surgical (overlay on rumen fistula), zootechnical (animal weight), statistics, spectrophotometer, pH meter, atomic absorption spectrophotometer AAS-30 apparatus Markhama. Novelty - the first time conducted comprehensive studies of the metabolic activity of the rumen by toxic heavy metals in the background feeding Sokyrnytskoho zeolite deposit. Stated that after 24-hour incubation of rumen contents by adding Pb, Cd, Cr (VI), compared with control in the incubation medium significantly reduced the amount of ammonia and VFA, cellulosolytic, proteolytic and amylase activity. Under the influence of heavy metal contents in the rumen decreases the ability of microorganisms to the splitting of the nutrient feed, reduced the intensity of the processes of fermentation and growth of microorganisms. Positive influence of sorbents, trace elements, vitamin E and B-carotene in populations of microorganisms bovine rumen contents in vitro by the action of Pb, Cd, Cr (VI). It was found that of sorbents - zeolite and Polysorb inhibitory effect of Cr (VI)> Cd> Pb weakened. Add to incubation microbial environment with heavy metals, trace elements - Fe, Cu, Se, Zn has a positive effect on growth and vital activity of microorganisms. Adding to the incubation medium selenite sodium, vitamin E and В-carotene has a positive effect on growth and enzymatic activity of different populations of rumen increases the number VFA, microbial mass increases cellulosolytic activity content scar. The effectiveness of implementation - Terms thesis introduced in the educational process and scientific research departments at Lviv National University of Veterinary Medicine and Biotechnology named after S. comparatively cheaper Lviv National Agrarian University, National University of Life and Nature. Scope: biochemistry, feeding. livestock.