Arkhypova K. The informativeness of EHF dielectrometry method in the determination of beta-adrenergic activity erythrocytes and platelets.

The changes in the complex permittivity of red blood cells and platelets induced by biologically active substances. The aim is to improve the EHF dielectrometry method for the study of functional state of both erythrocytes and platelets membranes, to establish a relationship between the dielectric characteristics of blood cells of their beta-adrenergic activity in health and disease. Methods of investigation are: EHF dielectrometry, method of changing the osmotic resistance of erythrocytes under the influence of beta-blockers, finite difference time domain and numerical methods. A new informative approach to the evaluation of beta-adrenergic activity of erythrocyte membranes has been developed. The dielectric properties of erythrocytes and platelets in health and cardiovascular diseases have been studied. It was found that the changes of dielectric parameters of blood cells induced by the adrenergic substances had depended on the physiological state of donors. It was detected a drug dose-dependent nature of permittivity under the effect of doxorubicin with blood cells of rats. A new algorithm for calculation of complex permittivity for the new type of waveguide sensor with improved characteristics was developed. The measurement accuracy of high-loss biological fluids has been increased. Fields of application: biotechnology, medicine, pharmacology.