Object: effects of tumor necrosis factor, expressed gelatinous nucleus, the nerves and perineural tissue structural changes in them under the influence of drugs of biological therapy. Objective: To examine the influence of drugs, inhibitors of tumor necrosis factor alpha in the conservative treatment of herniated discs animals and humans. Methods: The morphology of electron microscopy and morphometry to study the structure of nerve and surrounding tissues; Experimental animal modeling to create a model, clinical and radiological using magnetic resonance and computed tomography for the diagnosis and evaluation of conservative treatment of herniated discs in patients osteochondrosis. The study represents a new direction in the study of mechanisms of inflammation in the nerve and perineural tissue under his contact with disc herniation. In the experimental modeling herniated disc set that transplantation gelatinous nucleus (as part of the herniated disc) on the nerve leads to pronounced inflammation and destructive changes in its structure and perineural tissues. This inflammation and swelling, accompanied by demyelination of nerve fibers, damage to axons observed in the early postoperative period and are of lasting nature. The disturbances of the shape variability diameter myelin, bezmiyelinovyh nerve fibers and myelin destruction layer. Destructive changes such as thinning and lysis neyrofilamenty recorded in bezmiyelinovyh fibers. In shvanivskyh cells showed signs of destruction, accompanied by high heterohromatyzatsiyeyu cell nucleus. In connective tissues and m'yazoviyi develops pronounced inflammation with a tendency towards chronicity. For the first time in experiments on animals conducted correction inflammatory and destructive disorders of the nervous tissue, caused by the action of cytokines expressing jelly nucleus implanted in the area of nerve agents by using biological therapy - adalimumaba and infliksimaba. It is shown that under the action of these drugs reduces swelling, inflammation in the nerve and perineural tissues, which contributes to the delay destruction of nerve tissue. However, destructive changes in peripheral nerves before treatment on the background effects of tumor necrosis factor-alpha after gelatinous nucleus of rats on a nerve, though there have been, but were significantly reduced compared with the control group animals. Determined that the metabolic parameters that characterize the acute phase of inflammation were reduced after treatment of animals and infliksimabom adalimumabom than psevdooperovanymy animals. Revealed that the ratio of glycoproteins, sialic acid, haptoglobin, and the index value of neutrophils and lymphocytes in the patients in the treatment infliksimabom can be used to characterize the inflammatory. Experimental research is the basis for the use of the developed model to study various options for biological therapy in the conservative treatment of herniated disc, aimed at inhibiting the biosynthesis of tumor necrosis factor alpha and other proinflammatory cytokines. The use biological therapy drugs for conservative treatment. It is proved that biological therapy drugs are potentially effective in the case of herniated discs chronic genesis associated mainly with inflammation and swelling of tissue spinal canal, and ineffective or questionable in the case of large sekvestrovanyh disc herniation traumatic. The results introduced into clinical practice "Institute of Spine and Joint Pathology .prof. Sitenko NAMS of Ukraine ", Transkarpational Regional Clinical Hospital. A. Novak, Institute of injury SHEE "Uzhgorod National University" MONmolodsportu Ukraine and in the educational process relevant departments of the National Medical University, Ministry of Health of Ukraine, Lugansk State Medical University, Ministry of Health of Ukraine, SHEE "Uzhgorod National University" MONmolodsportu Ukraine. Traumatology and orthopedics.
