Fediakov R. Biochemical mechanisms of action of aflatoxin B1 on erytropoiesis in white rats

Object - aflatoxicosis, erythropoiesis, oxygen-blood function, biochemical mechanisms of aflatoxicosis and their correction. The purpose - to determine the effect of aflatoxin B1 (AFB1) on the function of blood oxygen transport and metabolism of red blood cells, the elucidation of metabolism in the cells of the kidney (as an organ of the excretory system and the site of synthesis of erythropoietin) and spleen of rats in experimental aflatoxicosis, the possibility of adjusting metabolic antioxidants (drug "E-selenium") and the use of yeast Phaffia rhodozyma. Methods: biochemical (spectrophotometric), cytological, statistical. Novelty: first analyzed the biochemical mechanisms of action of aflatoxin B1 on the functional activity of red blood cells in rats. For the first time defined the role of the activation of the formation of reactive oxygen species (ROS) and lipid peroxidation (LPO) in the mechanisms of toxicity AFB1 oxygen transport and functional activity of the kidneys and spleen. The first was shown that the level of oxidative stress caused by the action of AFB1, expressed in kidney cells than in spleen cells. Analyzed metabolic abnormalities in one-time intraperitoneal injection of AFB1 doses of 0.5 mg / kg and 0.25 mg / kg and a long oral dose of 0.025 mg / kg for 14 days in the red blood cells, kidney cells and spleen. The first time that the use of biomass yeast Phaffia rhodozyma and the drug "E-Selenium" reduces the toxic effects of aflatoxin B1 on erythropoiesis and antioxidant system in animal cells. Results: at different doses and methods of exposure of rats aflatoxin B1 suppresses erythropoiesis violates oxygen transport function of blood, leads to the development of oxidative stress in red blood cells, kidney cells and spleen. Under the influence of aflatoxin B1 reduced resistance to acid hemolysis of red blood cells, increases the permeability of the plasma membrane of cells of other organs and tissues, which is accompanied by an increase of enzyme activity (aminotransferase, lactate dehydrogenase), and creatinine levels in plasma and decreasing ratio between the activity of aspartate and alanine aminotransferase. Experimentally shown corrective action of the drug "E-Selenium" and yeast biomass P. rhodozyma on the performance of erythropoiesis, antioxidant system, the intensity of lipid peroxidation in erythrocytes, kidney and spleen tissues of laboratory rats exposed to AFB1.