Semochko O. The functioning of Na+, K+-pump and lipid peroxidation process in the embryos loach under electromagnetic radiation

The dissertation is devoted to the action of electromagnetic radiation visible spectrum on functioning of prooxidant-antioxidant homeostasis and Na+, K+- ATPase during the early embryogenesis of loach Misgurnus fossilis L. It was shown that dose-dependent effect of radiation caused intensification of lipid peroxidation, incrising of enzyme activity antioxidant system of embryos. We detected that light of blue and green range inhibits activity Na+, K+-ATPase. The influence of monochromatic light depends on the stage of embryo development, the wave of radiation, and sensitivityof systems to light. Results of correlation analysis indicated that blue and green light caused violation of relationships in the systems of prooxidant-antioxidant homeostasis and ion transport in cells of loach embryos. The activation of LPO, AOS enzymes, and inhibition of ATPase have been confirmed by ultrastructural changes in loach embryos. The action of light invoke next changes of organelles as an increasing of hialoplazmy density, the number of secondary lysosomes, reducing the tubules aEPR, destruction of membranes in mitochondria. This is result of metabolic disorders. The hypothetical scheme of light action has been proposed.