Rostama S. Molecular and cellular mechanisms of Dunaliella viridis Teodor. adaptation to high concentrations of copper

The object of study: Сopper sensitive and copper-resistant culture of microalgae Dunaliella viridis Teodor. var. viridis f. euchlora, strain IBASU-A N 29 and the laboratory Wistar rats. Purpose: Study the effects of multiple sequential effects of various toxic concentrations of copper sulphate on the metabolism of nucleic acids and some indicators of pro-and antioxidant system in the culture of Dunaliella viridis and the relationship of biochemical changes with cellular response to these changes. Methods: Spectrophotometry, method of tracer isotopes, atomic absorption spectrometry, the microscopic method, polarography, statistical analysis. Theoretical and practical results, novelty: Was shown that the sequential multiple introduction of copper sulphate culture Dunaliella viridis or intraperitoneal injectionto animals, accompanied by the formation of stability both in animals and in plants to lethal doses of toxicant. Founded that the hormesis effect ,that support adaptation of organism to toxic compounds depends on the schema and their preliminary dose introductions. For the first time was shown the correlation between the change epigenotype and behavior of cells in culture (loss of mobility, the formation of aggregates). And shown that heavy metals ions can be separated into two groups by the ability to form cell aggregates in culture of cell microalgae Dunaliella viridis: induced and non-induced aggregation. Shown that different metals, such as copper, lead and cadmium could effect differently on the immobilization of cells and the process of formation of cell aggrerates. For the first time on a model-resistant culture of Dunaliella viridis proved the reversibility of the induced aggregation of cells and proved their role in the adaptation of cells to toxic concentrations of heavy metals. Knowledge of the mechanisms of adaptation to the conditions of cultivation is great practical importance. The features observed loss of cell motility Dunaliella viridis in the presence of various metal ions depends on the various doses and time, makes it possible not only to determine the boundaries of the toxicity of various substances, but also to detect the presence of various toxic compounds in the aquatic environment.Field of application and performance The identified relationship between the functional activity of the genome, motility and the formation of cell aggregates allows the use of culture Dunaliella viridis as a cellular biosensor.