Basarab I. Changes of activity of marker enzymes in blood and animal organs under effect of exogenous factors

Object - metabolism of carbohydrate and lipids in animals with disorders of the digestive system. Purpose - to examine peculiarities and regularities of changes of the activity of marker enzymes, their specific molecular isoforms and content of key intermediates in the blood and liver cells under conditions of harmful, protective and renewable effect of exogenous factors. Methods: biochemical (enzymatic, electrophoretic, chromatographic, spectrophotometric, ultracentrifugation), statistics. Novelty: on an experimental model of the accelerated development alcohol-induced hepatic steatosis in rats, it has been obtained new data of peculiarities and regularities of changes in activity of marker enzymes and many biochemical parameters of blood and liver cells under conditions of harmful effect of exogenous ethanol and protective action of phospholipid-containing additives of vegetable (soy) and animal (milk) origin in capsular and liposomal form. It has been shown that the phospholipid-containing additives of milk origin in liposomal form have the highest protective ability. Preventive and restorative use it in combination with the traditional protection under the conditions of development of the digestive system disorders of alimentary origin in neonatal calves can accelerate normalization of metabolic processes in the organism by 20-30% and significantly restore content of important intermediates in the blood and hepatocyte compartments. For determining the level of disorders of the digestive system and its protection and restoration of calves, it is recommended to use isozyme of lactate dehydrogenase - LDG5 of liver cells as the real molecular. It has been carried out a physiological and biochemical research in area of the study of regularities of changes of activity of marker enzymes, their specific molecular isoforms and content of key intermediates in the blood and cells of animal organs of digestive system under effect of harmful, protective and renewable exogenous factors. In an experimental model of the accelerated development alcohol-induced hepatic steatosis in rats, it has been studied regularities of changes in activity of marker enzymes and many biochemical parameters of blood and liver cells under conditions of harmful effect of exogenous ethanol (EtOH) and protective action of phospholipid-containing additives of vegetable (soy) and animal (milk) origin in capsular and liposomal form. It has been shown that the phospholipid-containing additives of milk origin in liposomal form have the highest protective ability. Preventive and restorative use it in combination with the traditional protection under the conditions of development of the digestive system disorders of alimentary origin in neonatal calves can accelerate normalization of metabolic processes in the organism by 20-30% and significantly restore content of important intermediates in the blood and hepatocyte compartments. The real diagnostic marker for determining the level of disorders of the digestive system and its protection and restoration of calves could be isozyme of lactate dehydrogenase - LDG5 of liver cells.