Vishchur V. Fatty acids exchange and heavy metals contents in bees within different intensities of technogenic load on the environment.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0413U006785

Applicant for

Specialization

  • 03.00.04 - Біохімія

19-11-2013

Specialized Academic Board

Д.35.368.01

Essay

Object - the exchange of fatty acids and heavy metals in tissues of honey bees , depending on the intensity of environmental impact . The aim of the thesis was to clarify the influence of different intensity environmental impact on the content of fatty acids and heavy metals in tissues of honey bees and bee products. Methods: Biochemical ( spectrographic , chromatographic ) , statistics (mean, error of the mean , the probability of the difference between the average values ??). The novelty of the studied parameters characterizing the exchange of heavy metals, anionic and nonetherified fatty acids , and fatty acids of total lipids in bee pollen , honey bee tissues and svizhopobudovanyh honeycomb (tongue ) derived from beehives that are located in areas with different intensity of technogenic load . Under such conditions studied concentration of heavy metals , including toxic in natural Polyphlore meads . There were likely reduction of iron, zinc, copper , nickel, cadmium and Pb concentrations and increased nonetherified fatty acids and fatty acids of total lipids in bee pollen , honey bee tissues and honeycomb (tongue ), which were from hives that were placed in the low intensity of technogenic load. Under these conditions, in natural Polyphlore meads reduced levels of heavy metals , as confirmed by the patent of Ukraine № 70079 . Results: alongside the reduction of intensity of technogenic load on the environment the dandelion and apple tree pollen, tissues of the bee abdomen, chest and head, just built honeycombs underwent the reduction of anionic fatty acids, but the level of non-etherified fatty acids and fatty acids of common lipids increased. Moreover, the content of anionic and non-etherified fatty acids in bee pollen, tissues of the bee abdomen, just built honeycombs changed due to saturated fatty acids with even and odd number of carbon atoms in the chain, monounsaturated fatty acids of n-7 and n-9 families and polyunsaturated fatty acids of n-3 and n-6 families.

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