Zaburannyi N. Structural genomics of elements that regulate the biosynthesis of bacterial transglycosylase antibiotic inhibitors

The research has helped to identify the novel operator sites for pleiotropic regulators of secondary metabolism within moe- and tcp-clusters, to investigate the role of gene tcp28 in the biosynthesis of teicoplanin and to develop specialized software for the analysis of codon-dependent (translational) regulation in bacteria, in particular actinomycetes. As a result of bioinformatic analysis, by detailed annotation of regulatory genes of S. ghanaensis, we identified a number of orthologues of regulatory proteins from S. coelicolor. Models of regulatory networks which involve these elements were proposed. We discovered that Tcp28 is a positive path-specific regulator of the synthesis of teicoplanin. These results for the first time reveal regulatory mechanisms that are involved in controlling the synthesis of teicoplanin. In the course of the work we obtained the strain with increased production T-A2. The Web service for analysis and visualization of relative intragenic codon location at genome-wide level was created.