Object of research: The formation of the biocenosis of tick-borne bacterial zooanthroponoses infections and circulation of these pathogens in biotic objects with different floro-faunistic complexes, including the population of the investigated area. Aim: To find out the range of circulating bacterial natural foci especially dangerous pathogens (EDP) in biotic environmental objects that form the parasitic tick -borne zooanthroponoses system different on different floro-faunistic complexes in Kyiv region, and to assess the medical importance of topical EDP by comprehensive microbiological studies of the potential these pathogens reservoirs and vectors, and population on these area. Metods: bacteriological, microscopy, serological, indirect hemagglutination reaction, enzyme immunoassay, agglutination test, molecular genetics , a biological, zoological, field experimental in natural habitats, and statistics. As a result of long-term observations, had been identified of vectors and animals - potential reservoirs of transmissible tick EDP, first revealed circulation of B. burgdorferi sensu lato in Kyiv region among mainly Ixodes ricinus ticks, less of two Dermacentor genus ticks; C. burnetii - I. ricinus ticks, Dermacentor reticulatus; F. tularensis - all members of the family Ixodidae and small rodents - Mus musculus, Apodemus agrarius. According to integrated laboratory monitoring F. tularensis was relevant to Kiev region at the present time, as well as the first to demonstrate the circulation of C. burnetii and the rapid increasing of the ranges were detected to B. burgdorferi sensu lato. Francisella tularensis was circulated in the system "small mammals: mouse houses, field mouse, and mouse baby - family Ixodidae ticks ". The most common pathogens were B. burgdorferi sensu lato. The circulation of Borrelia afzelii and Borrelia garinii was first established in the Kiev region by typing of genus. The functioning of combined foci of pathogens on region was first proved with prevailed association of B. burgdorferi sensu lato - A. phagocytophilum. The circulation of C. burnetii in natural and anthropurgic foci in Kiev region by the results of the antigen detection as well as the genome of the pathogen in ticks first was proved. First time the circulation of A. phagocytophilum and Ehrlichia spp. were proved by the real-time PCR studies. Serological testing of human blood serum with fever state confirmed the relevance of B. burgdorferi sensu lato, C. burnetii to shaping the region's population morbidity. First the suitability of foreign manufactured reagents had been assessed that are used to identify of B. burgdorferi sensu lato and F. tularensis strains from Kiev region. The expediency of the usage of the kits for real-time PCR " DNA RealBest Borrelia burgdorferi sensu lato (set 2/RG)" ("Vector-Best", Russia) for detection of Borrelia pathogenic complex B. burgdorferi sensu lato. Odject of research: medical microbiology, laboratory diagnostics, epizootic and epidemiological monitoring.
