Krotevych M. Morphological types of papillary thyroid cancer and prognosis of the disease

The thesis is devoted to the improvement of the efficiency of diagnosis, treatment optimization and prediction of papillary thyroid cancer from a study of the major prognostic factors of its histological variants. Basis of morphological and molecular biological research thesis made cytological and histological preparations of 413 patients operated on papillary thyroid cancer. To achieve the tasks used clinical laboratory, cytological, histopathological, macroscopic study, quantitative polymerase chain reaction (PCR), DNA extraction, statistical research. The basic trends in the incidence and progression of histological variants of papillary thyroid cancer and their relationship with the pathogenic factors. First set of clinical and morphological prognostic criteria for high risk of recurrence, metastasis of thyroid cancer, invasion of surrounding tissues and blood vessels. High efficiency and appropriateness of Fine needle aspiration biopsy (FNAВ) with cytology in the diagnosis of histological subtypes of papillary cancer. It is proved that stained cytological preparations FNAВ thyroid nodes can be used for DNA isolation and subsequent evaluation of the presence of BRAF mutation by PCR. Determined that the mutation detection data is of limited value for the diagnosis of malignancy in cases of suspicious cytological findings FNAВ, and in cases of malignant FNAВ findings may be useful for preoperative determination of tumor at high risk extra thyroid invasion and presence of metastases in lymph nodes.