Chuienko A. Micromycetes as agents of damage of the rubber technical articles

Thesis for a candidate of biological sciences degree by speciality 03.00.07 - microbiology. - D.K. Zabolotny institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv, 2014. The dissertation is devoted to the studying of ecological and physiological peculiarities of micromycetes that expand at rubber technical articles. From RTA it was isolated 192 strains that were related to 36 species of 20 genera of microscopic fungi. For the first time ever it has been shown the ability of some micromycetes to the growth in the inner (15-25 mm) layers of the rubber by using of the confocal laser scan microscopy.On the base of data of the interrelations between microscopic fungi A. alternata, A. flavus, A.sydowii, A. ustus, C. cladosporioides, C. sphaerospermum, F. poae, P. chrysogenum P. expansum and T. viride was shown the possibility of employment of them as test-cultures for the checking of funginertness of RTA. It has been established that strains of these microscopic fungi, isolated from rubber substrates, were characterized by increased indexes of the velocity of radial growth on the media that contained caoutchouc as source of carbon (CCM) and carboxylesterase activity in comparision with the strains, isolated from natural substrates. Estimation of influence of micromycetes on rubber technical articles, that included definition of ball of their funginertness, condition of the surface and blend composition by IR spectroscopy method, shown that influence of microscopic fungi, isolated from damaged RTA was more significant than one's that recommended by standard GOST 9.049-91. It has been shown effectiveness of using as fungicide the oligomer derivant of guanidine М1 that contained propane-2, 2-diyldibenzene chemical group, in concentration 3% for the defense of RNA from fungal spoiling.