Savko U. Biochemical mechanisms of rat duodenal epitheliocytes damage upon long-term suppression of gastric hydrochloric acid secretion

The thesis deals with the study of dysbiotic-inflammatory pathway role in damage of rat duodenal epitheliocytes upon long-term suppression of gastric hydrochloric acid secretion. The following parameters were determined in the study: intensity of free-radical processes, indices of duodenum antioxidative system state, expression level of few genes involved in development of inflammation, hypertrophy, regeneration and response to pathogenic microbiota of duodenal epitheliocytes upon dysbiosis and normobiosis in rats with chronically suppressed gastric acid secretion. Results of performed investigations indicate that following biochemical mechanisms are involved in duodenal cells damage upon selected experimental conditions: violation of prooxidative/antioxidative balance and shift in expression pattern of few studied genes in duodenal cells. It was elucidated that damage level in crypt epithelial cells was higher in comparison with villus epithelial cells of rats with chronically suppressed gastric acid secretion. The leading role of dysbiotic-inflammatory pathway in damage of rat duodenal epitheliocytes upon long-term suppression of gastric hydrochloric acid secretion was established.