Vislovukh A. The regulatory role of untranslated regions of eukaryotic translation elongation factor 1A mRNA

Thesis is devoted to the regulatory role of untranslated regions of the mRNA EEF1A and control of mechanisms of their expression at the posttranscriptional level. For the first time obtained experimental data on the presence of posttranscriptional control of EEF1A mRNAs expression, particularly during the differentiation of myoblasts and in sublines of breast cancer cell lines of varying degrees of malignancy. It was shown that despite the high level of homology, eEF1A1 and eEF1A2 proteins are equally stable, whereas EEF1A1 and EEF1A2 mRNAs differ significantly in their half-life time. For the first time it was shown that the PTI-1 mRNA potentially encodes a peptide that is homologous to the hormone relaxin, overexpression of which is observed in cases of prostate cancer. It was suggested that the oncogenic effect of PTI-1 is realized via relaxin-mediated pathway. For the first time, it was shown that the EEF1A2 expression is regulated at the post-transcriptional level by two miRNAs, miR-663 and miR-744. It was found that miRNA-mediated downregulation of EEF1A2 leads to inhibition of cell proliferation of breast cancer cells MCF7. For the first time, were obtained data that indicate that the reduced expression of protooncogene EEF1A2, due to the action of one of the components of red wine resveratrol, is miRNA-mediated.