Sakevych V. The role of polymorphisms Toll-like receptors 2,4 and Galectin-10 in the development of allergic rhinitis

In the thesis the theoretical generalizations and new research assignment solution of Immunology and Allergology concerning the definition of genetic determinism in allergic rhinitis by examining the role of Toll-like receptors of innate immunity by polymorphisms TLR2 (rs5743708), TLR 4 (rs4986790) and CLC-10 (rs420297) in pathogenesis and analysis of relationships between genotype and haplotype of patients immune status indicators and clinical course of the disease are given. At examined patients allergic rhinitis in 76 % cases is predominantly of hereditary nature from the mother (36%). During the study of the immune status of patients with allergic rhinitis in 15 % of patients eosinophilia was observed, increase ofthe average level of total immunoglobulin E, increase at the relative number of CD4+CD25+Foxp3+ Тrеg cells with the reducing amount of IL -10 and increase IL -4. Revealed significant differences between the frequencies of genotypes in the control group and patients with allergic rhinitis gene polymorphism by TLR 4 (Asp299Gly)(р = 0,03). Рolymorphism rs420297 of the gene CLC- 10 was significantly more common in patients with allergic rhinitis(p = 0,04). The significant association between the presence of polymorphic alleles of the gene CLC- 10 levels and CD4+revealed, CD4+ CD25+, in the group of homozygous carriers of polymorphic alleles T and C alleles of the gene CLC- 10. The authentic association between the presence of polymorphic alleles of the gene CLC- 10 levels and CD4+CD25+Foxp3+, CD4+. It is established, that individuals, who carry the gene polymorphic allele CLC- 10 have significantly higher levels of IgE, IL -4 and lower levels of IL10. At patients with allergic rhinitis haplotype carriers TLR4 / CLC 10 containing polymorphic alleles G and T. Significantly higher level of expression of molecules CD4+CD25+Foxp3+ Тrеg cells(p=0,024) of reducing the amount of IL -10 and increasing IL-4 is found.