Kucherenko A. Allelic polymorphism of cytokine genes (IL6, IL8, IL10 and IFNL4) in patients with monogenic disorders and mass pathology

Object - molecular genetic factors of hereditary susceptibility to ischemic stroke and recurrent pregnancy loss, recurrent corneal erosion caused by lattice dystrophy and individual response to PEG-interferon therapy of chronic hepatitis C. Aim - to establish IL6, IL8, IL10 and IFNL4 gene polymorphic variants role as factors of genetic susceptibility to ischemic stroke, disturbance of homeostasis in the mother-foetus system, and recurrent erosion development in patients with hereditary corneal stromal dystrophy, as well as genetic factors of individual response to therapy of chronic viral hepatitis C. Methods - isolation and purification of genomic DNA, polymerase chain reaction, restriction fragment length polymorphism analysis, sequencing, bioinformatic and statistical analysis. Results and their novelty - DNA bank of individuals with varying effectiveness of chronic hepatitis C treatment was created. Allelic variants IL6 gene -174C, IL8 gene -781C and IL10 gene -592A are established as modifiers of phenotype in patients with lattice corneal dystrophy. Carrier status of the IL8 gene -781T and IL10 gene -592C alleles is a factor of susceptibility to ischemic stroke. IL10 gene -592 CC genotype may be a marker of an improvement prognosis for ischemic stroke patients within first two weeks of treatment. IL10 gene -592AA genotype and -1082A allele are factors of genetic susceptibility to miscarriage. Genotype comprising IL6 -174G and ESR1 -397C alleles in the homozygous state may be regarded as a genetic marker of successful pregnancy maintenance during early gestation. Association of IFNL4 gene ss469415590 polymorphism with treatment efficacy in patients with chronic hepatitis C has been proved. Field - molecular genetics.