Ponomarenko S. Biological properties of Staphylococcus aureus strains isolated from different biotopes, and optimization of laboratory diagnostics of staphylococcal infection

Object of the research: biological properties of S.aureus strains of different origin; growth and selective properties of culture media for cultivation of staphylococci. The aim - optimize the laboratory diagnostics of a staph infection on the basis of the new data about the features of the biological properties of S.aureus strains, isolated from different biotopes, by the mean of constructing of the new elective medium from plant material. Equipment: microscope, thermostat, autoclave, dry-heat oven, spectrophotometer, laboratory centrifuge, single-channel pipettes of variable volume, laboratory scales, auto reader. It was shown that 66.1% of S.aureus strains isolated from hospital objects of environment were highly adhesive, and 53.2% had a high capacity for biofilm formation. Scientific ideas about the persistence factors of S.aureus clinical strains were widen: the indices of their antilyzosyme and anticomplementary activities were in the range of high-level and intermediate-level and respectively. For the first time it was theoretically and experimentally proved appropriateness of vegetable raw materials for hydrolysates and fermentative lysates production. The technologies of the nutritive base obtaining from treacle fermentative lysate, grain distillers, barley pellet; from the acid hydrolysates of treacle, barley pellet were developed. The degree of implementation: 5 patents, 1 application for a utility model in Ukraine, 5 technologies, 3 acts of implementation. Scope (area) of application: medicine (microbiology, bacteriology, biotechnology).