Vus K. Fluorescent detection of amyloid fibrils

The object of study: interaction of new fluorescent probes with amyloid fibrils. Aim of work: elucidating the physical detail of interaction between new benzanthrone, squaraine dyes and Thioflavin T derivatives and amyloid fibrils of varying morphology. Methods: fluorescence spectroscopy, spectrophotometry, transmission electron microscopy, circular dichroism, quantum-chemical methods. Scientific novelty: a broad characterization of the association of novel fluorescent dyes with lysozyme and insulin amyloid fibrils was given. The thermodynamical parameters of fluorophore binding with fibrils have been obtained, the dye location in the protein aggregates has been characterized and a positive correlation between the sensitivity of the most promising benzanthrones to amyloid lysozyme and quantum-chemical descriptors of lipophilicity, dye width and the sum of nitrogen charges was obtained. Practical meaning: application in molecular biophysics, pharmacology, molecular biology, medicine and combinatorial chemistry.