Zabarna I. Veterinary and sanitary expertise of products of broiler chickens' slaughter in a case of formazine and tylocyclinvet usage

For the first time ever it was carried out a methodic validation for a determination of tylosin in poultry meat and egg samples using an enzyme-linked immunosorbent assay approach according to the requirements of ISO17025 standard and Decision 657/2002/EC. We have also conducted a monitoring on determining the residual amounts of tylosin and doxycycline in poultry muscles and eggs (2009?2015) and can state about an absence of positive results obtaining. It was determined that the biggest amount of tylosin residues was found in a middle part of an iliotibial tract and a liver in occasional samples, which had come in the trading environment. The amount of doxycycline, which was found only in the middle part of iliotibial tract, did not increase a maximal allowable limit. Using of such preparations as formazine and tylocyclinvet with medical and preventive purposes has a positive effect on a live weight increment. According to the microbiological characteristics and meat toxicity of broiler chickens we have found that formazine strongly accumulates in «white» muscles and tylocyclinvet – in «red» muscles. Biological value of meat in a case of formazine usage tends to decline and in a case of tylocyclinvet usage – to improve. In order to detect tylosin residues in the broiler chicken bodies using a liquid chromatography method it is necessary to sample the widest back muscles, the middle part of the iliotibial tract, the caudal iliac and the acetabular muscle. For the detection of doxycycline residues a muscular part of a stomach must be researched.