Babets Y. The distribution of neurospecific proteins of the heart, blood plasma and brain in rats under doxorubicin toxicity and antioxidant protection effects

The thesis focused on the quantitative distribution of neuron and astrocyte-specific proteins and hyaluronan-binding activity of extracellular matrix proteins in the brain, heart and blood plasma under doxorubicin injection. The doxorubicin in a dose of 1 mg/kg promoted a number of biochemical changes to induced cardiomyopathy in rats. First time, it was founded the redistribution of soluble and membrane forms of neuronal molecules of cell adhesion depending on the brain areas: an increase sNCAM in the thalamus and mNCAM - in the cerebellum under the impact of doxorubicin in a dose of 1 mg/kg during 4 weeks, which can lead to changes of intercellular adhesion in these regions of the brain. An increase of NCAM level was founded in the PNS of heart, which indicated an activation of synaptic plasticity under doxorubicin impact. It has been shown a slight increase of the level of calcium-binding protein S-100b in the hippocampus, thalamus and the cerebral cortex that induces redistribution filament form of glial fibrillary acidic protein in the hippocampus and cerebellum under the effect of doxorubicin. It has been shown the latest evidence of glycosaminoglycan binding proteins sensitivity in the brain and heart under doxorubicin intoxication. It was shown that the conditions of doxorubicin-induced cardiomyopathy in the heart induced the decrease of the S-100b level, which leads to a significant increase (almost 2 times) of filament GFAP level. Partially prevention of significant impact to the redistribution of neuron specific proteins were noticed under doxorubicin effect in complex with ketoglutarate or korvitin that improved of physiological characteristics of animals, but wasn't shown completely protection from the doxorubicin-induced intoxication