Shton I. Efficacy of gold nanoparticles application for anticancer photodynamic therapy (experimental research)

The thesis is devoted to the in vitro and in vivo study of chlorin e6 composites with gold nanoparticles (NPs) of different geometry and aspect ratios (nanospheres, spherical-like nanoparticles, nanoshells, nanoprisms and nanorods) as photosensitizers for antitumor photodynamic therapy. It was shown that the chlorin e6 interaction with spherical-like gold NPs led to bathochromic shift of its long wavelength absorption maximum of about 34 nm, and chlorine e6 interaction with gold nanospheres - of 9 nm. Singlet oxygen generation rate of photosensitizer composites with spherical-like gold NPs, gold nanoshells and nanoprisms in model solutions inversely correlated with gold content in composite. Gold nanoprisms and nanoshells aggregated on the surface of cell lines Namalwa and MT-4 and did not interact with normal human lymphocytes. In vitro photodynamic activity of chlorin e6 composites with gold nanospheres (15nm), nanoprisms and spherical-like gold NPs increased 1,6-4,4 times comparatively to free photosensitizer activity. It was revealed that chlorine e6 composites with nanospheres (15nm), spherical-like gold NPs and nanoprisms possessed high antitumor photodynamic activity in vivo on mice with transplanted Lewis lung carcinoma. The highest antitumor photodynamic activity among tested preparations was established with the use of composite photosensitizers that comprised spherical or spherical-like gold NPs.