Tokarchuk K. Aldehydes participation in oxidative stress development in vitro and in vivo

Object: oxidative stress development not only in Fenton’s reaction and after aldehyde influence on rat’s thymocytes but also in expereimental rhabdomyolysis and streptozotocin-induced diabetes in rats. Aim: to clarify the aldehyde role in oxidative stress development with aldehyde acceptor dimedone using. Methods: preparative biochemistry, enzymatic methods, spectrophotometry, spectrofluorimetry, ELISA, EPR-spectroscopy, methods of light microscopy and flow cytometry, methods of mathematical statistics. It was investigated aldehydes role in oxidative stress development in vitro and in vivо with acceptor dimedone using. It was demonstrated aldehyde participation in oxidative stress development in Fenton’s system (Fe2+-ascorbic acid) in rat’s thymocytes and in experimental models of rhabdomyolysis and streptozotocin-induced diabetes in rats. Besides, it was shown that such aldehydes as formaldehyde, glyoxal, methylglyoxal and acrolein can induce oxidative stress in rat’s thymocytes. It was proved dimedone ability to decrease aldehyde content in rat’s thymocytes. It was demonstrated the normalization of carbonyl and oxidative stress parameters in different tissues after dimedone using. Dimedone has not toxic effect for rats in dose 50 mg/kg body weight.