Iarmoliuk Y. Influence of tissue transplantation on angiogenesis in the zone of brain ischemic injury in experiment

The thesis is dedicated to study the effects of stimulated angiogenesis in the zone of ischemic brain injury using intracerebral embryonic nervous tissue (TENT) and bone marrow tissue transplantation (BMTT) on the modified intraluminal middle cerebral artery occlusion (MCAO) with blocking collateral blood flow in rats. 204 white non-bred male rats at the age of 3-4 months weighing 280-320 g, were divided into experimental groups and subgroups based on the performed procedure: MCAO, MCAO and TENT, MCAO and BMTT, MCAO with subsequent intracerebral infusion of phosphate-buffered 0,9% normal saline (MCAO and PBS) on the 2 day after MCAO, with sham-oсclusion of the middle cerebral artery (sham-MCAO) and intracerebral injection on the 2-nd day after sham-MCAO. The volume of brain infarction (VBI) was determined by staining brain slices with 2,3,5 - triphenyltetrasolium chloride on the 7 and 14 days after MCAO; and the number of vessels in the ischemic boundary zone (IBZ) - using immunohistochemical detection of СD34 in endothelial cells on the 1 and 3 (comparison between MCAO and sham-MCAO groups), 7, 14 and 28 day (comparison between other groups) after MCAO. The neurologic assessment and histopathologic study was performed on the 1, 3, 7, 14, 21 and 28 days after MCAO. We found that BMTT and ТENT caused the progressive increase in the vessel count in IBZ of brain in rats, being most prominent on the 28 day after MCAO, decrease of the volume of brain infarction and neurologic improvement from the 3 to the 28 day of observation. BMTT proved to be more efficient than TENT in neurologic restoration in experimental rats due to its angiogenic effects.